The properties file is a human-readable configuration file which provides the configuration details for the collected data. To start a Tracer session, start the Tracer version of the CellProfiler Analyst application (available here), which will request a properties file. Questions? Please direct them to our online forum dedicated to CellProfiler Analyst. Make sure to install the 64-bit Windows version, and then add the JDK bin location to your PATH environment variable ( instructions). The Java Development Environment (JDK) is required for Tracer to run download from here. If you already have CellProfiler Analyst installed on your system, it is recommended to install the Tracer version to a different folder. Tracer is a modification of the CellProfiler Analyst package. We have leveraged all of these capabilities into a new package, CellProfiler Tracer. Lastly, we have developed CellProfiler Analyst for the purpose of data exploration. In addition, we have included object tracking functionality in our CellProfiler open-source software. The Imaging Platform at the Broad Institute specializes in producing the rich set of measurements characteristic of these screening efforts. In particular, for high-content screening purposes, where the biologist is looking at a large number of per-cell measurements, it is vital to be able to visually assess the development of these measurements across time within the local context of the cell being examined. While a wide range of tracking algorithms have been developed, open-source tools tailored towards the visualization of their results, in particular the assessment of the result quality, are uncommon. Often, the dynamic behavior of organisms, cells, organelles, or molecular assemblies can only appreciated by observing them over time. In the world of cellular image analysis, time-lapse assays remain one of the more challenging domains in biology. Tracer is available within CellProfiler Analyst and enables visualization and quality assessment of cellular trajectories obtained via time-lapse imaging. This project is not currently maintained.ĬellProfiler Tracer is a data visualization and exploration tool for time-lapse image-based assays. ![]() ![]() A component of CellProfiler Analyst cell image analysis software
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